Channel Proteomes

Project

Native AMPA Receptor Complexes

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes

Project Overview

Name Native AMPA Receptor Complexes
Title

High-resolution proteomics unravel architecture and molecular diversity of native AMPA receptor complexes

Publication

http://dx.doi.org/10.1016/j.neuron.2012.03.034

Description

AMPA-type glutamate receptors (AMPARs) are responsible for a wide variety of processes in the mammalian brain including fast excitatory neurotransmission, postsynaptic plasticity or synapse development. Here we show by using comprehensive and quantitative proteomic analyses that native AMPARs are macromolecular complexes with an unappreciated large molecular diversity. This diversity results from co-assembly of the known AMPAR subunits, pore-forming GluA and three types of auxiliary proteins, with 21 newly identified constituents, mostly secreted proteins or transmembrane proteins of different classes. Their integration at distinct abundance and stability establishes the heteromultimeric architecture of native AMPAR complexes: a defined core with a variable periphery resulting in an apparent molecular mass between 0.6 and 1 MDa. Co-assembly of the newly identified constituents changes the gating properties of AMPARs and provides novel molecular links to the protein dynamics fundamental for the complex role of AMPARs in formation and operation of glutamatergic synapses.

Target Proteins

Protein Name Uniprot/SwissProt
GluA1 Glutamate receptor 1 (GluR-A) P19490
GluA2 Glutamate receptor 2 (GluR-B) P19491
GluA3 Glutamate receptor 3 (GluR-C) P19492
GluA4 Glutamate receptor 4 (GluR-D) P19493
View all proteins related to this project

Antibodies

Name Supplier
Anti-GluA1-a Millipore, USA
Anti-GluA1-b Synaptic Systems, Germany
Anti-GluA1-c Synaptic Systems, Germany
Anti-GluA2-A3 Millipore, USA
Anti-GluA2-a Millipore, USA
Anti-GluA2-b NeuroMab, USA
Anti-GluA2-c Santa Cruz Biotechnology, USA
Anti-GluA3 Synaptic Systems, Germany
Anti-GluA4-a Millipore, USA
Anti-GluA4-b Santa Cruz Biotechnology, USA
View detailed antibody list

Solubilization Buffers

Name Supplier
ComplexioLyte 47 Logopharm GmbH, Germany
ComplexioLyte 91 Logopharm GmbH, Germany